Difference between revisions of "Part:BBa K1104301:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1104301 short</partinfo>
 
<partinfo>BBa_K1104301 short</partinfo>
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===Design Notes===
 
===Design Notes===
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None
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==''Exons and Introns''==
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<p>CDS from exons:</p>
−
 
+
<p>exon1: atgaaaatctattttattgtcggccttctcttcatggctatggttgctatcatggctgcacctgttg</p>
 +
<p>exon2: aggatgaattcgagccacttgagcattttgagaatgaagaacgtgccgacagacatagaagagtaacttgtgaccttctctcattcaaaggacaagttaatgacagtgcttgcgctgctaactgtctcagtttgggtaaagctggaggtcattgcgagaaaggagtttgtatttgtcgaaa</p>
 +
<p>exon3: aaccagtttcaaagatctctgggacaaacgtttcggttaa</p>
 +
 +
<p>mRNA from exons</p>
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<p>exon1 (001-137):</p> <p>gtcagacacttctcagtcttttttcgaatagtttgaatatctattgaagtttaacacgtatttagccaatatgaaaatctattttattgtcggccttctcttcatggctatggttgctatcatggctgcacctgttg</p>
 +
<p>exon2 (138-318):</p> <p>aggatgaattcgagccacttgagcattttgagaatgaagaacgtgccgacagacatagaagagtaacttgtgaccttctctcattcaaaggacaagttaatgacagtgcttgcgctgctaactgtctcagtttgggtaaagctggaggtcattgcgagaaaggagtttgtatttgtcgaaa</p>
 +
<p>exon3 (319-680):</p>
 +
<p>aaccagtttcaaagatctctgggacaaacgtttcggttaagtgccattatgaagattcaacgatgaaattttttaacaaaaaatatgaaaacgcgaaatgattaagaaatgaaaaatgaaaaactgtattatcattgaaaattctatgaactttaatttacttatttaataaataatataagtaaattatcttttggt
 +
ttaatttctttaaagtattatcttttgaataaataaatttttttatatagataaattagataataatagaaaataaattttttttctaaatatactaattattatattcattattttaataattaaattgttaaatgcagtaattaacaatagaaatttaaaaa</p>
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 +
<p>material peptide based on mRNA sequence (200 to 355)</p>
 +
<p>exon2:</p>
 +
<p>8887754</p>
 +
<p>gtaacttgtgaccttctctcattcaaaggacaagttaatgacagtgcttgcgctgctaactgtctcagtttgggtaaagctggaggtcattgcgagaaaggagtttgtatttgtcgaaa</p>
 +
<p>8887872</p>
 +
<p>exon3:</p>
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<p>8888151</p>
 +
<p>aaccagtttcaaagatctctgggacaaacgtttcggt</p>
 +
<p>8888187</p>
 +
 +
[[File:NYMU_Group 5 Defensin 1.png|1000px]]
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 +
*See more:[http://www.ncbi.nlm.nih.gov/gene/406143 http://www.ncbi.nlm.nih.gov/gene/406143]
 +
 +
==''Defensin1 primers(example)''==
 +
ATGGTAACTTGTGACCTTCTCTCATTCA
 +
          TGTGACCTTCTCTCATTCAAAGGACAAGTTAATGACAGTGCTTGCGCTGCTAACTGTCT
 +
                                                    GCGCTGCTAACTGTCTCAGTTTGGGTAAAGCTGGAGGTCATTGCGAGAAAGGAGTTTGT                                                                                               
 +
                                                                                              GCGAGAAAGGAGTTTGTATTTGTCGAAAAACCAGTTTCAAAGATCTCTGGGACAAACGTTTCGGTTAA
 +
              19nt,51C                                  16nt,52C                                    17nt,50C
  
 
===Source===
 
===Source===

Latest revision as of 07:47, 5 October 2013

Defensin1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Exons and Introns

CDS from exons:

exon1: atgaaaatctattttattgtcggccttctcttcatggctatggttgctatcatggctgcacctgttg

exon2: aggatgaattcgagccacttgagcattttgagaatgaagaacgtgccgacagacatagaagagtaacttgtgaccttctctcattcaaaggacaagttaatgacagtgcttgcgctgctaactgtctcagtttgggtaaagctggaggtcattgcgagaaaggagtttgtatttgtcgaaa

exon3: aaccagtttcaaagatctctgggacaaacgtttcggttaa

mRNA from exons

exon1 (001-137):

gtcagacacttctcagtcttttttcgaatagtttgaatatctattgaagtttaacacgtatttagccaatatgaaaatctattttattgtcggccttctcttcatggctatggttgctatcatggctgcacctgttg

exon2 (138-318):

aggatgaattcgagccacttgagcattttgagaatgaagaacgtgccgacagacatagaagagtaacttgtgaccttctctcattcaaaggacaagttaatgacagtgcttgcgctgctaactgtctcagtttgggtaaagctggaggtcattgcgagaaaggagtttgtatttgtcgaaa

exon3 (319-680):

aaccagtttcaaagatctctgggacaaacgtttcggttaagtgccattatgaagattcaacgatgaaattttttaacaaaaaatatgaaaacgcgaaatgattaagaaatgaaaaatgaaaaactgtattatcattgaaaattctatgaactttaatttacttatttaataaataatataagtaaattatcttttggt ttaatttctttaaagtattatcttttgaataaataaatttttttatatagataaattagataataatagaaaataaattttttttctaaatatactaattattatattcattattttaataattaaattgttaaatgcagtaattaacaatagaaatttaaaaa

material peptide based on mRNA sequence (200 to 355)

exon2:

8887754

gtaacttgtgaccttctctcattcaaaggacaagttaatgacagtgcttgcgctgctaactgtctcagtttgggtaaagctggaggtcattgcgagaaaggagtttgtatttgtcgaaa

8887872

exon3:

8888151

aaccagtttcaaagatctctgggacaaacgtttcggt

8888187

NYMU Group 5 Defensin 1.png

  • See more:[http://www.ncbi.nlm.nih.gov/gene/406143 http://www.ncbi.nlm.nih.gov/gene/406143]

Defensin1 primers(example)

ATGGTAACTTGTGACCTTCTCTCATTCA
         TGTGACCTTCTCTCATTCAAAGGACAAGTTAATGACAGTGCTTGCGCTGCTAACTGTCT 
                                                    GCGCTGCTAACTGTCTCAGTTTGGGTAAAGCTGGAGGTCATTGCGAGAAAGGAGTTTGT                                                                                                
                                                                                              GCGAGAAAGGAGTTTGTATTTGTCGAAAAACCAGTTTCAAAGATCTCTGGGACAAACGTTTCGGTTAA
              19nt,51C                                   16nt,52C                                    17nt,50C

Source

Apis melliferra

References