Difference between revisions of "Part:BBa K1217003"
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[[File:HKU13_KOppkSDS.png|400px|thumb|none|SDS-PAGE analysis of expression of K.oralis PPK1 enzyme inside E.coli. Expression of PPK1 is induced by 0.5mM IPTG at 30 degree for 5 hours. Band marked with star is the PPK1 enzyme, the enzyme expressed is soluble. ]] | [[File:HKU13_KOppkSDS.png|400px|thumb|none|SDS-PAGE analysis of expression of K.oralis PPK1 enzyme inside E.coli. Expression of PPK1 is induced by 0.5mM IPTG at 30 degree for 5 hours. Band marked with star is the PPK1 enzyme, the enzyme expressed is soluble. ]] | ||
| − | Efficiency of this construct is compared with Two other | + | Efficiency of this construct is compared with Two other designs [https://parts.igem.org/Part:BBa_K1217008 BBa_K1217008] and [https://parts.igem.org/Part:BBa_K1217010 BBa_K1217010]. |
| + | The | ||
==='''Efficiency in Poly-P synthesis'''=== | ==='''Efficiency in Poly-P synthesis'''=== | ||
Revision as of 05:20, 5 October 2013
pT7-ppk1(K.oralis)
Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217003, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria.
Usage and Biology
Expression of ppk1 enzyme
Efficiency of this construct is compared with Two other designs BBa_K1217008 and BBa_K1217010. The
Efficiency in Poly-P synthesis
Efficiency in phosphate removal from the environment
Testing
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 309