Difference between revisions of "Part:BBa K1197014"

Latest revision as of 04:07, 5 October 2013

House fly cytochrome P450 reductase (fCPR)

fCPR is a cytochrome P450 reductase found originally in Musca domestica (house fly). It is necessary for functional working of CYP6G1 as well as many other cytochrome P450 enzymes. It makes CYP6G1 enzyme catalytically active.

Reference: Andersen, J.F., Utermohlen, J.G., Feyereisen, R., 1994. Expression of house fly CYP6A1 and NADPH-cytochrome P450 reductase in Escherichia coli and reconstitution of an insecticide-metabolizing P450 system. Biochemistry 33, 2171e2177.

fCPR Production

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1247
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1769

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1197014 short</partinfo>
-fCPR is a cytochrome P450 reductase found originally in Musca domestica (house fly). It is necessary for functional working of CYP6G1 as well as many other cytochrome P450 enzymes. It makes CYP6G1 enzyme catalitically active.
+fCPR is a cytochrome P450 reductase found originally in Musca domestica (house fly). It is necessary for functional working of CYP6G1 as well as many other cytochrome P450 enzymes. It makes CYP6G1 enzyme catalytically active.
@@ Line 8: / Line 8: @@
 and NADPH-cytochrome P450 reductase in Escherichia coli and reconstitution of
 an insecticide-metabolizing P450 system. Biochemistry 33, 2171e2177.
+'''fCPR Production'''
+https://static.igem.org/mediawiki/parts/f/fd/FCPR.jpg
 <!-- Add more about the biology of this part here