Difference between revisions of "Part:BBa K1152016"
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<partinfo>BBa_K1152016 short</partinfo>
 
<partinfo>BBa_K1152016 short</partinfo>
  
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This engineered version of indC BBa_K11520013 contains a synthetic T-domain instead of its native T-domain. It still is capable to produce the blue pigment indigoidine, as OD-measurements showed.
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This engineered version of indC BBa_K11520013 contains a synthetic T-domain instead of its native T-domain. It still is capable to produce the blue pigment indigoidine as OD-measurements showed.
  
 
In the multiple sequence alignment showed below, this variant of indC is named synT1.
 
In the multiple sequence alignment showed below, this variant of indC is named synT1.

Revision as of 04:04, 5 October 2013

IndC Indigoidine Synthetase device synT1 (engineered)

This engineered version of indC BBa_K11520013 contains a synthetic T-domain instead of its native T-domain. It still is capable to produce the blue pigment indigoidine as OD-measurements showed.

In the multiple sequence alignment showed below, this variant of indC is named synT1.

Multiple Sequence Alignment of the synthetic T-domains we introduced to indC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2730