Difference between revisions of "Part:BBa K1202112:Experience"

 
Line 1: Line 1:
 +
_Signal Peptide experiments
 +
In this part of our project we tested are our signal peptides is working or not
 +
To secrete GFP protein via signal peptide to extracellular membrane.
 +
In overall project, our aim related with signal peptide is to secrete apoptotic proteins outer membrane. If this construct secrete GFP successfully, that means signal peptide works and TorA can be used in Apoptin or E4orf4 export. 
 +
And also we use constitutive promoter in our designs because we need high amount of protein and we want to our bacteria always produce our proteins.
 +
We made santrifuge our signal peptides’ (TorA-GFP ve GFP-HlyA) liquid culture falcons and then get supernatant from falcons. Then we measured values of absorption of GFP in our supernatant in suitable wavelength. Results show that we found high amount of GFP in our supernatants.
 +
Result;
 +
 +
Column scale(y)  shows us quantity amount of GFP in the solution. 
 +
 +
we get good results from our GFP-HlyA that we found high amount of GFP in our samples more than our Negative(-) Control.
 +
Another good results that our Positive Control which is GFP that added in LB showed maximum amount of GFP in graphics. So we can said that our controls were working too.
 +
  
−
__NOTOC__
 
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
−
how you used this part and how it worked out.
 
  
 
===Applications of BBa_K1202112===
 
===Applications of BBa_K1202112===

Revision as of 04:00, 5 October 2013

_Signal Peptide experiments In this part of our project we tested are our signal peptides is working or not To secrete GFP protein via signal peptide to extracellular membrane. In overall project, our aim related with signal peptide is to secrete apoptotic proteins outer membrane. If this construct secrete GFP successfully, that means signal peptide works and TorA can be used in Apoptin or E4orf4 export. And also we use constitutive promoter in our designs because we need high amount of protein and we want to our bacteria always produce our proteins. We made santrifuge our signal peptides’ (TorA-GFP ve GFP-HlyA) liquid culture falcons and then get supernatant from falcons. Then we measured values of absorption of GFP in our supernatant in suitable wavelength. Results show that we found high amount of GFP in our supernatants. Result;

Column scale(y) shows us quantity amount of GFP in the solution.

we get good results from our GFP-HlyA that we found high amount of GFP in our samples more than our Negative(-) Control. Another good results that our Positive Control which is GFP that added in LB showed maximum amount of GFP in graphics. So we can said that our controls were working too.


Applications of BBa_K1202112

User Reviews

UNIQa1424c137eb2677c-partinfo-00000000-QINU UNIQa1424c137eb2677c-partinfo-00000001-QINU