Difference between revisions of "Part:BBa K1216003:Design"
(→‎Design Notes)
 
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We obtain the plasmid from Johannes Haerle of the Panke Group from D-BSSE in Basel and sequence them for restriction sites and further cloning steps
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We obtain the plasmid with the hydrolase from Johannes Haerle of the Panke Group from D-BSSE ETHZ in Basel, checked it for biobrick restriction sites and removed them. We sequenced them to confirm the sequence and did PCR with overlapping fragments to introduce the biobrick pre and suffix to clone them into biobrick vectors.
  
 
===Source===
 
===Source===

Latest revision as of 03:53, 5 October 2013

beta-N-Acetylglucosaminidase (nagZ) from Escherichia Coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 25
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 76


Design Notes

No illegal restriction sites.



We obtain the plasmid with the hydrolase from Johannes Haerle of the Panke Group from D-BSSE ETHZ in Basel, checked it for biobrick restriction sites and removed them. We sequenced them to confirm the sequence and did PCR with overlapping fragments to introduce the biobrick pre and suffix to clone them into biobrick vectors.

Source

E.coli

References