Difference between revisions of "Part:BBa K950002"
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<img src="https://static.igem.org/mediawiki/2013/d/d5/TueDefiziente_staemme.png" style="width: 600px; border: solid 1px black;">  
 
<img src="https://static.igem.org/mediawiki/2013/d/d5/TueDefiziente_staemme.png" style="width: 600px; border: solid 1px black;">  
  
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<p> Basal expression level of mOrange under the control of the promotors Pfet3, <b>Panb1</b> and Psuc2. Non-transformed yeast was used as negative control. Fluorescence was detected using a RFP filter set (ET Bandpass 470/40, ET Bandpass 572/35). </p>
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<p>Increased promoter activity of Psuc2 and Panb1 in repressor deficient strains. Fluorescence was detected using a RFP filter set (ET Bandpass 470/40, ET Bandpass 572/35).</p>
  
 
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<img src="https://static.igem.org/mediawiki/2013/8/83/TuePanb_Psuc_deficient_strains.png" style="width: 600px; border: solid 1px black;">  
 
<img src="https://static.igem.org/mediawiki/2013/8/83/TuePanb_Psuc_deficient_strains.png" style="width: 600px; border: solid 1px black;">  
  
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<p> Basal expression level of mOrange under the control of the promotors Pfet3, <b>Panb1</b> and Psuc2. Non-transformed yeast was used as negative control. Fluorescence was measured using a plate reader (Ex: 548nm +/-9nm, Em: 581nm +/-20nm) and normalized to OD600 of the cell suspension. All measurements were performed as triplicates. </p>
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<p>Promoter activity of Psuc2 and Panb1 in repressor deficient strains. The promoter activity was enhanced by a factor of 2.6 for Psuc2 and 13.8 for Panb1. Fluorescence was measured using a plate reader (Ex: 548nm +/-9nm, Em: 581nm +/-20nm) and normalized to OD600 of the cell suspension. All measurements were performed as triplicates. </p>
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Revision as of 03:46, 5 October 2013

yeast anb1 promotor

This is the promotor of yeast anb1 gene. Panb1 is a target of the rox1 repressor, see Part BBa_K950001


 

Increased promoter activity of Psuc2 and Panb1 in repressor deficient strains. Fluorescence was detected using a RFP filter set (ET Bandpass 470/40, ET Bandpass 572/35).

 

Promoter activity of Psuc2 and Panb1 in repressor deficient strains. The promoter activity was enhanced by a factor of 2.6 for Psuc2 and 13.8 for Panb1. Fluorescence was measured using a plate reader (Ex: 548nm +/-9nm, Em: 581nm +/-20nm) and normalized to OD600 of the cell suspension. All measurements were performed as triplicates.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 124
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]