Difference between revisions of "Part:BBa K1216003:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1216003 short</partinfo>
 
<partinfo>BBa_K1216003 short</partinfo>
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No illegal restriction sites.
 
No illegal restriction sites.
  
 
+
<br><br>
 +
We obtain the plasmid from Johannes Haerle of the Panke Group from D-BSSE in Basel and sequence them for restriction sites and further cloning steps
  
 
===Source===
 
===Source===

Revision as of 03:44, 5 October 2013

beta-N-Acetylglucosaminidase (nagZ) from Escherichia Coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 25
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 76


Design Notes

No illegal restriction sites.



We obtain the plasmid from Johannes Haerle of the Panke Group from D-BSSE in Basel and sequence them for restriction sites and further cloning steps

Source

E.coli

References