Difference between revisions of "Part:BBa K1197012"

 
 
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<partinfo>BBa_K1197012 short</partinfo>
 
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P-coumaric acid production:
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The aim of modeling P-coumaric Acid production is to predict the amount and the activity of this enzyme ,Sam8, and the production of  p-coumaric acid. The model was created mainly by designing ODEs and enzyme kinetics equations .Then; these equations were solved by the help of MATLAB software.
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In our circuit, Sam8 gene is transcribed with B. subtilis specific constituve promoter Pveg.  SacB is transcribed with Sam8 gene for secretion out of cell.
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• We have calculated our enzyme activity according to our enzyme production rate. In 3500 s ,  12E9 molecule of p-coumaric acid is produced according to our model.
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'''Sam8 activity:'''
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https://static.igem.org/mediawiki/parts/2/2d/P-coumaric_acid.jpg
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'''Sam8 amount'''
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https://static.igem.org/mediawiki/parts/c/c4/Sam8.jpg
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References:
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1- https://static.igem.org/mediawiki/2009/6/6c/ODEs.pdf
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:38, 5 October 2013

Pveg + RBS + Sam 8 + DT

P-coumaric acid production: The aim of modeling P-coumaric Acid production is to predict the amount and the activity of this enzyme ,Sam8, and the production of p-coumaric acid. The model was created mainly by designing ODEs and enzyme kinetics equations .Then; these equations were solved by the help of MATLAB software.

In our circuit, Sam8 gene is transcribed with B. subtilis specific constituve promoter Pveg. SacB is transcribed with Sam8 gene for secretion out of cell.


• We have calculated our enzyme activity according to our enzyme production rate. In 3500 s , 12E9 molecule of p-coumaric acid is produced according to our model.


Sam8 activity:

P-coumaric_acid.jpg

Sam8 amount

Sam8.jpg

References:

1- https://static.igem.org/mediawiki/2009/6/6c/ODEs.pdf

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1053
    Illegal BamHI site found at 1455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 796
    Illegal AgeI site found at 743
    Illegal AgeI site found at 912
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 347
    Illegal BsaI site found at 613