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| − | ===In vitro characterization of BBa_K1132031 ===
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| − | '''Conception'''
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| − | The basic idea is to perform the switch with a cell lysis containing the recombinase and the plasmid containing the gate to be switched. After incubation, transformation of the plasmid containing the gate allows the quantification of switched versus non switched plasmids. Experiments have been performed as described in the protocol.
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| − | [[http://2013.igem.org/Team:INSA_Toulouse/contenu/lab_practice/notebook/protocols/charac_recomb]]
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| − | '''Results'''
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| − | In the absence of recombinase, the colonies are white. As a result, AND gate does not have transcriptional leakage and non-specific recombinases cannot switch the door.
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| − | https://static.igem.org/mediawiki/2013/1/14/A1.PNG
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| − | We tested the AND gate with Bxb1. After incubation with Bxb1, the colonies are still white (this is normal!), therefore visual inspection of the switch was not possible. The only possibility was to sequence independent clones We analyzed five independent colonies by sequencing, all of them were switched.
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| | ===User Reviews=== | | ===User Reviews=== |
Latest revision as of 03:35, 5 October 2013
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