Definition of different domain border combinations for T-domain exchanges
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The figure shows a sequence alignment of the indC and bpsA amino acid sequences.
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The alignment was created using clustalO (http://www.ebi.ac.uk/Tools/msa/clustalo/) with
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standard parameters. The lines marked A, B and C reflect the borders we used between the A-
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and the T-domain, whereas those marked, 1, 2, 3 and 4 reflect the borders between the T- and
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the TE-domain. In total we tried all twelve combinations of a domain border {A, B, C} and a
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domain border {1, 2, 3, 4}, replacing the sequence inbetween with the respective part of bpsA.
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b) E. coli TOP10 co-transformed with modified versions of indC and the PPTase sfp
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The co-tranformation of the modified indC-(bpsA-T) plasmids described above with a second
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plasmid coding for the PPTase Sfp shows that only three domain border combinations can be
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used for exchanging the indC T-domain with the T-domain of bpsA. These are the
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combinations A1, A2 and C1. We applied combination A2 for further T-domain exchanges.
Revision as of 03:02, 5 October 2013
IndC Indigoidine Synthetase device
This part encodes the non-ribosomal peptide synthetase that synthesizes the blue pigment indigoidine. The IndC gene was amplified from P. luminescens laumondii TT01 (DSM15139) and cloned into pSB1C3.
