Difference between revisions of "Part:BBa K606040:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
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===Applications of BBa_K606040===
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The Hyperspank promoter was designed to be compatible for B. subtilis. It is shorter than the classic one, but is also recognised by E. coli polymerases.
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This promoter Hyper Spanck is IPTG inducible.
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It has been characterised in front of a tRNA amber that would be used in the translation of a GFP amber.
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We characterised it with an RFP expression system (PHs-RBS-RFP), into TURBO cells that express LacI to allow the promoter to be the less leacky possible. This system is inducible by IPTG.
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<br><br>
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Without IPTG<br><br><br>
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{| border="1" class="wikitable" style="text-align: center;"
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|E.coli containing Hyperspank+RBS+RFP - IPTG at 37°C
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|-
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|[[Image:minusfluo1.jpg|450px|thumb|center|-IPTG E.coli at 37°C  (rfp image)]]
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|[[Image:minustrans1.jpg|450px|thumb|center|-IPTG E.coli at 37°C  (trans image)]]
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|-
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|[[Image:minusfluo2.jpg|450px|thumb|center|-IPTG E.coli at 37°C  (rfp image)]]
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|[[Image:minustrans2.jpg|450px|thumb|center|-IPTG E.coli at 37°C  (trans image)]]
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|}
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<br>
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With IPTG : <br><br>
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{| border="1" class="wikitable" style="text-align: center;"
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|E.coli containing Hyperspank+RBS+RFP + IPTG at 37°C
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|-
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|[[Image:hovigfluo1.jpg|450px|thumb|center|+IPTG E.coli at 37°C  (rfp image)]]
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|[[Image:hovigtrans1.jpg|450px|thumb|center|+IPTG E.coli at 37°C  (trans image)]]
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|-
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|[[Image:hovigfluo2.jpg|450px|thumb|center|+IPTG E.coli at 37°C  (rfp image)]]
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|[[Image:hovigtrans2.jpg|450px|thumb|center|+IPTG E.coli at 37°C  (trans image)]]
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|}
  
 
===User Reviews===
 
===User Reviews===
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<partinfo>BBa_K143064 AddReview 5</partinfo>
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This part was used as a part of the Bee subtilis, iGEM METU 2013 project. We simulated the production of RFP under two different IPTG concentrations to observe its basal and maximum transcription rates.
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<!-- DON'T DELETE --><partinfo>BBa_K606040 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K606040 EndReviews</partinfo>

Latest revision as of 02:39, 5 October 2013


The Hyperspank promoter was designed to be compatible for B. subtilis. It is shorter than the classic one, but is also recognised by E. coli polymerases.

We characterised it with an RFP expression system (PHs-RBS-RFP), into TURBO cells that express LacI to allow the promoter to be the less leacky possible. This system is inducible by IPTG.



Without IPTG


E.coli containing Hyperspank+RBS+RFP - IPTG at 37°C
-IPTG E.coli at 37°C (rfp image)
-IPTG E.coli at 37°C (trans image)
-IPTG E.coli at 37°C (rfp image)
-IPTG E.coli at 37°C (trans image)


With IPTG :

E.coli containing Hyperspank+RBS+RFP + IPTG at 37°C
+IPTG E.coli at 37°C (rfp image)
+IPTG E.coli at 37°C (trans image)
+IPTG E.coli at 37°C (rfp image)
+IPTG E.coli at 37°C (trans image)

User Reviews

UNIQ2318ccade4487e22-partinfo-00000000-QINU

•••••

This part was used as a part of the Bee subtilis, iGEM METU 2013 project. We simulated the production of RFP under two different IPTG concentrations to observe its basal and maximum transcription rates.

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UNIQ2318ccade4487e22-partinfo-00000002-QINU