Difference between revisions of "Part:BBa K1152013"

 
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<partinfo>BBa_K1152013 short</partinfo>
 
<partinfo>BBa_K1152013 short</partinfo>
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Nonribosomal Peptide Synthetase that synthesizes the blue pigment indigoidine. The gene was amplified from ''P. luminescens laumondii'' TT01 (DSM15139).
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The indC indigoidine synthetase BBa_K1152008 is under a lac promoter BBa_R0010 and the RBS BBa_B0034.  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1152013 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1152013 SequenceAndFeatures</partinfo>
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[[image:20130925_indBB_dig13.png|400px|Restriction digest of BBa_K1152013 with EcoRI, XbaI, SpeI and PstI]]
  
 
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Revision as of 02:16, 5 October 2013

IndC Indigoidine Synthetase device Nonribosomal Peptide Synthetase that synthesizes the blue pigment indigoidine. The gene was amplified from P. luminescens laumondii TT01 (DSM15139).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4087
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1467
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2730

Restriction digest of BBa_K1152013 with EcoRI, XbaI, SpeI and PstI