Difference between revisions of "Part:BBa K1197013"

 
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__NOTOC__
 
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<partinfo>BBa_K1197013 short</partinfo>
 
<partinfo>BBa_K1197013 short</partinfo>
  
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It is an insecticide resistance gene which is found in Drosophila Melanogaster. We used it in our system to metabolize imidacloprid by producing nontoxic byproducts.  
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It is an insecticide resistance gene which is found in ''Drosophila melanogaster''. We used it in our system to metabolize imidacloprid by producing nontoxic byproducts.  
  
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It is obtained from Drosophila Melanogaster genome and modified according to work in B.subtilis.
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It is obtained from ''Drosophila melanogaster'' genome and modified according to work in ''B.subtilis''.
  
 
Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991)
 
Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991)
  
 
Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity
 
Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity
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of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl.
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of recombinant cytochrome P450 17a-hydroxylase in ''Escherichia coli''. Proc. Natl.
 
Acad. Sci. USA 88, 5597e5601.
 
Acad. Sci. USA 88, 5597e5601.
  

Revision as of 02:04, 5 October 2013

CYP6G1 - Insecticide resistance gene

It is an insecticide resistance gene which is found in Drosophila melanogaster. We used it in our system to metabolize imidacloprid by producing nontoxic byproducts.

It is obtained from Drosophila melanogaster genome and modified according to work in B.subtilis.

Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991)

Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl. Acad. Sci. USA 88, 5597e5601.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 542
    Illegal XhoI site found at 1210
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 943