Difference between revisions of "Part:BBa K1155003"
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In order to be easily used by all the other teams, our team improved the biobrick BBa_K592009 by assembly of biobricks B0034, BBa_K592009 and B0015 to construct a composite part including a ribosome binding site, full length of amilCP gene and double terminator. We used it as one of our reporter gene in ''Escherichia coli''.
 
In order to be easily used by all the other teams, our team improved the biobrick BBa_K592009 by assembly of biobricks B0034, BBa_K592009 and B0015 to construct a composite part including a ribosome binding site, full length of amilCP gene and double terminator. We used it as one of our reporter gene in ''Escherichia coli''.
 +
 +
We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3
 +
 +
BBa_K1155000 is a promoteur Pndh* repressed in anaerobic condition by binding of the FNR regulator.(see BBa_K1155000 for more information)
 +
 +
What we expected :
 +
 +
+ O2 : FNR inactivated : Pndh* activated --> amilCP expression --> purple color
 +
 +
- O2 : FNR activated : Pndh* repressed --> amilCP not expressed --> no color
 +
 +
What we observed :
 +
 +
+ O2 : --> purple color
 +
 +
- O2 : --> no color
 +
 +
[photo]
 +
 +
These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:03, 5 October 2013

RBS-amilCP-term

AmilCP is a blue/purple chromoprotein from coral Acropora millepora. This protein exhibits a strong colour that we can visualize by naked eyes. The biobrick part (BBa_K592009) which including amilCP gene was created by Team Uppsala - Sweden 2011.

In order to be easily used by all the other teams, our team improved the biobrick BBa_K592009 by assembly of biobricks B0034, BBa_K592009 and B0015 to construct a composite part including a ribosome binding site, full length of amilCP gene and double terminator. We used it as one of our reporter gene in Escherichia coli.

We have tested the Biobricks BBa_K1155003 in the construction: BBa_K1155000 + BBa_K1155003 in pSB1C3

BBa_K1155000 is a promoteur Pndh* repressed in anaerobic condition by binding of the FNR regulator.(see BBa_K1155000 for more information)

What we expected :

+ O2 : FNR inactivated : Pndh* activated --> amilCP expression --> purple color

- O2 : FNR activated : Pndh* repressed --> amilCP not expressed --> no color

What we observed :

+ O2 : --> purple color

- O2 : --> no color

[photo]

These results indicate that the composite part BBa_K1155003 composed of a RBS + amilCP + terminator works very well and can be easily used as a reporter system of promoteur expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]