Difference between revisions of "Part:BBa K1197004"

Revision as of 01:54, 5 October 2013

RBS SpoVG + LacI + DT

This is a LacI producing part which is proper for B.subtilis. Any promoter can be added to the downstream of that part to obtain a LacI coding device.

This part is obtained from BBa_I732820 part by changing the original RBS sequence with RBS SpoVG via specific primers. And LacI was codon optimized for expression in B.subtilis. Original Elowitz RBS does not work well in B.subtilis according to literature researches.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Gel Electrophoresis photo of LacI construct (RBS SpoVG + LacI + DT)

@@ Line 4: / Line 4: @@
 This is a LacI producing part which is proper for B.subtilis. Any promoter can be added to the downstream of that part to obtain a LacI coding device.
-This part is obtained from BBa_I732820 part by changing the original RBS sequence with RBS SpoVG via specific primers. And LacI was codon optimized for expression in B.subtilis.
+This part is obtained from BBa_I732820 part by changing the original RBS sequence with RBS SpoVG via specific primers. And LacI was codon optimized for expression in B.subtilis. Original Elowitz RBS does not work well in B.subtilis according to literature researches.
@@ Line 14: / Line 14: @@
 <partinfo>BBa_K1197004 SequenceAndFeatures</partinfo>
+'''Gel Electrophoresis photo of LacI construct (RBS SpoVG + LacI + DT)'''
+https://static.igem.org/mediawiki/parts/9/95/MazF_RBS_LacI_DT.jpg
 <!-- Uncomment this to enable Functional Parameter display