Difference between revisions of "Part:BBa K1197005"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1197005 short</partinfo>
 
<partinfo>BBa_K1197005 short</partinfo>
  
−
.
+
MazF is a non-specific ribonuclease coding gene is used as killing agent against Bacillus subtilis. In our system, MazF is produced constitutively under Pveg promoter.
 +
 
 +
When MazF construct (Pveg + RBS SpoVG + MazF + RBS SpoVG + LacI + DT) was assemled in B.subtilis integration vector, BBa_K823023, we saw colonies when it is transformed into E.coli with Ampicillin resistance. On the other hand, we did not see any colony when the same plasmid was transformed into B.subtilis. Thus, it can be said that MazF construct is working in B.subtilis functionally.
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 11: Line 13:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1197005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1197005 SequenceAndFeatures</partinfo>
 +
 +
'''Gel electrophoresis photo of MazF construct:'''
 +
 +
https://static.igem.org/mediawiki/parts/1/1f/MazF_%2B_RBS_SpoVG_%2B_LacI_%2B_DT.jpg
  
  

Latest revision as of 01:46, 5 October 2013

MazF +RBS (SpoVG) + LacI + DT

MazF is a non-specific ribonuclease coding gene is used as killing agent against Bacillus subtilis. In our system, MazF is produced constitutively under Pveg promoter.

When MazF construct (Pveg + RBS SpoVG + MazF + RBS SpoVG + LacI + DT) was assemled in B.subtilis integration vector, BBa_K823023, we saw colonies when it is transformed into E.coli with Ampicillin resistance. On the other hand, we did not see any colony when the same plasmid was transformed into B.subtilis. Thus, it can be said that MazF construct is working in B.subtilis functionally.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Gel electrophoresis photo of MazF construct:

MazF_%2B_RBS_SpoVG_%2B_LacI_%2B_DT.jpg