Difference between revisions of "Part:BBa K1075020"
 
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__NOTOC__
 
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<partinfo>BBa_K1075020 short</partinfo>
 
<partinfo>BBa_K1075020 short</partinfo>
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===construction===
 
  
 
The part contains the mutated E. coli(ec)ssrA(DAS+4) tag.
 
The part contains the mutated E. coli(ec)ssrA(DAS+4) tag.
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===biology===
 
===biology===
  
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The ecssrA tag is a short peptide sequence, which is fused to the C-terminus of proteins, which should be degraded. It relates to the protease ClpXP complex in E.coli and it also allows the adaptor protein ecSspB binding and delivering substrates to the proteases in order to make the process more efficient. The mutated ecssrA (DAS+4) weakens a direct binding between proteases and ecssrA(DAS+4) and increases the dependance of ecSspB. [http://www.ncbi.nlm.nih.gov/pubmed/16762842]
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The (ec)ssrA tag is a short peptide sequence that can be fused to the C-terminus of proteins which should be degraded. The adaptor protein SspB binds to it and delivers the substrates to the protease ClpXP complex in E. coli. The mutation (DAS+4) in (ec)ssrA prevents the degradation of the ssrA-tagged protein without the presence of sspB and therefore makes the system more specific and controllable. [http://www.ncbi.nlm.nih.gov/pubmed/16762842]
  
 
===application===
 
===application===
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The ecssrA(DAS+4) tag can be fused to proteins, so they get degraded. The tag can be used by everyone e.g. to research the function of a protein.
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The (ec)ssrA(DAS+4) tag can be fused to proteins so that they will be delivered to the ClpXP protease and degraded.  
  
  

Latest revision as of 01:11, 5 October 2013

E. coli ssrA(DAS+4)

The part contains the mutated E. coli(ec)ssrA(DAS+4) tag.

biology

The (ec)ssrA tag is a short peptide sequence that can be fused to the C-terminus of proteins which should be degraded. The adaptor protein SspB binds to it and delivers the substrates to the protease ClpXP complex in E. coli. The mutation (DAS+4) in (ec)ssrA prevents the degradation of the ssrA-tagged protein without the presence of sspB and therefore makes the system more specific and controllable. [http://www.ncbi.nlm.nih.gov/pubmed/16762842]

application

The (ec)ssrA(DAS+4) tag can be fused to proteins so that they will be delivered to the ClpXP protease and degraded.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]