Difference between revisions of "Part:BBa K1075020"
 
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<partinfo>BBa_K1075020 short</partinfo>
 
<partinfo>BBa_K1075020 short</partinfo>
  
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fused to the C terminus of a protein it triggers degradation upon induction of sspB function.
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The part contains the mutated E. coli(ec)ssrA(DAS+4) tag.
  
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===biology===
  
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===Usage and Biology===
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The (ec)ssrA tag is a short peptide sequence that can be fused to the C-terminus of proteins which should be degraded. The adaptor protein SspB binds to it and delivers the substrates to the protease ClpXP complex in E. coli. The mutation (DAS+4) in (ec)ssrA prevents the degradation of the ssrA-tagged protein without the presence of sspB and therefore makes the system more specific and controllable. [http://www.ncbi.nlm.nih.gov/pubmed/16762842]
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The ssrA tag is a sequence, which allows proteolytic enzymes to degrade them. It relates to the protease ClpXP complex in E.coli and it also allows adaptor proteins such as sspB binding and delivering substrates to the proteases in order to make the process more efficient.
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The mutation ssrA (DAS+4) prevents a direct binding to the protease and ensures the dependance of sspB.
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===application===
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The (ec)ssrA(DAS+4) tag can be fused to proteins so that they will be delivered to the ClpXP protease and degraded.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1075020 SequenceAndFeatures</partinfo>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 01:11, 5 October 2013

E. coli ssrA(DAS+4)

The part contains the mutated E. coli(ec)ssrA(DAS+4) tag.

biology

The (ec)ssrA tag is a short peptide sequence that can be fused to the C-terminus of proteins which should be degraded. The adaptor protein SspB binds to it and delivers the substrates to the protease ClpXP complex in E. coli. The mutation (DAS+4) in (ec)ssrA prevents the degradation of the ssrA-tagged protein without the presence of sspB and therefore makes the system more specific and controllable. [http://www.ncbi.nlm.nih.gov/pubmed/16762842]

application

The (ec)ssrA(DAS+4) tag can be fused to proteins so that they will be delivered to the ClpXP protease and degraded.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 40
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]