Difference between revisions of "Part:BBa K1202109"

 
 
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<partinfo>BBa_K1202109 short</partinfo>
 
<partinfo>BBa_K1202109 short</partinfo>
  
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This is our another promoter system which is working in conjunction with LsR pLsr promoter system.
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Lsr system consists of three components, mainly. Lsr promoter, LsrR repressor and LsrK enzyme. This enzyme works as phosphorylase that catalyzes AI-2 phosphorylation reactions. AI-2-phosphorus complex allows the inhibition of repression effect of LsrR on Lsr promoter and provides the production of desired protein. Via this system, we want to regulate the production of our cancer killing peptides.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 00:56, 5 October 2013

LsrK Generator

Lsr system consists of three components, mainly. Lsr promoter, LsrR repressor and LsrK enzyme. This enzyme works as phosphorylase that catalyzes AI-2 phosphorylation reactions. AI-2-phosphorus complex allows the inhibition of repression effect of LsrR on Lsr promoter and provides the production of desired protein. Via this system, we want to regulate the production of our cancer killing peptides.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1517
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 108
    Illegal AgeI site found at 462
    Illegal AgeI site found at 624
    Illegal AgeI site found at 717
    Illegal AgeI site found at 902
    Illegal AgeI site found at 987
    Illegal AgeI site found at 1476
  • 1000
    COMPATIBLE WITH RFC[1000]