Difference between revisions of "Help:BioBrick Assembly"

Revision as of 14:33, 7 August 2006

Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on restriction enzymes, purification, ligation, and transformation - with a twist: BioBrick parts are composable. The result of assembling two parts is a new part that may be used in future assemblies. Certain RBS-CDS issues must also be considered.

Standard Assembly

BioBrick parts can be assembled to form useful devices, through a process often referred to as 'Standard Assembly'. This uses normal cloning techniques based on restriction enzymes, purification, ligation, and transformation. Find out more about Standard Assembly.

Parallel Assembly

BioBrick systems may contain many parts. One could spend many weeks building a 50-part system by assembling the first two parts, adding the third part, adding the fourth part, and so on. However, because BioBrick assembly is composable, assembly need not be done sequentially - Find out more...

Automated Assembly

Automated assemblies, currently in late stage development at iGEM labs. find out more here.

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-Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on restriction enzymes, purification, ligation, and transformation - with a twist: BioBrick parts are [[Assembly:Composable|composable]].  The result of assembling two parts is a new part that may be used in future assemblies.
+Physical parts in the DNA Repository have been designed to be assembled into systems using normal cloning techniques based on restriction enzymes, purification, ligation, and transformation - with a twist: BioBrick parts are [[Assembly:Composable|composable]].  The result of assembling two parts is a new part that may be used in future assemblies. Certain [[Assembly:RBS-CDS issues|RBS-CDS issues]] must also be considered.
 ==Standard Assembly==