Difference between revisions of "Part:BBa K1202011"

 
 
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<partinfo>BBa_K1202011 short</partinfo>
 
<partinfo>BBa_K1202011 short</partinfo>
  
 
LsrK is an intracellular enzyme that phosphorylates autoinducer-2 (AI-2) quorum sensing unit. This reaction ends up with the inhibition of LsrR repression on Lsr promoter. This allows the production of coding region under the promoter.  
 
LsrK is an intracellular enzyme that phosphorylates autoinducer-2 (AI-2) quorum sensing unit. This reaction ends up with the inhibition of LsrR repression on Lsr promoter. This allows the production of coding region under the promoter.  
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Lsr system consists of three components, mainly. Lsr promoter, LsrR repressor and LsrK enzyme. This enzyme works as phosphorylase that catalyzes AI-2 phosphorylation reactions. AI-2-phosphorus complex allows the inhibition of repression effect of LsrR on Lsr promoter and provides the production of desired protein. Via this system, we want to regulate the production of our cancer killing peptides.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:37, 4 October 2013

LsrK

LsrK is an intracellular enzyme that phosphorylates autoinducer-2 (AI-2) quorum sensing unit. This reaction ends up with the inhibition of LsrR repression on Lsr promoter. This allows the production of coding region under the promoter.

Lsr system consists of three components, mainly. Lsr promoter, LsrR repressor and LsrK enzyme. This enzyme works as phosphorylase that catalyzes AI-2 phosphorylation reactions. AI-2-phosphorus complex allows the inhibition of repression effect of LsrR on Lsr promoter and provides the production of desired protein. Via this system, we want to regulate the production of our cancer killing peptides.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 61
    Illegal AgeI site found at 415
    Illegal AgeI site found at 577
    Illegal AgeI site found at 670
    Illegal AgeI site found at 855
    Illegal AgeI site found at 940
    Illegal AgeI site found at 1429
  • 1000
    COMPATIBLE WITH RFC[1000]