Difference between revisions of "Part:BBa K1150042"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1150042 short</partinfo> | <partinfo>BBa_K1150042 short</partinfo> | ||
| − | . | + | {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right" |
| + | ! colspan="2" style="background:#FFBF00;"|RNaimer (Bla target 4) | ||
| + | |- | ||
| + | |'''Function''' | ||
| + | |Targeting VEGF4 with Cas9 | ||
| + | |- | ||
| + | |'''Use in''' | ||
| + | |Mammalian cells | ||
| + | |- | ||
| + | |'''RFC standard''' | ||
| + | |[https://parts.igem.org/Help:Assembly_standard_25 RFC 25] | ||
| + | |- | ||
| + | |'''Backbone''' | ||
| + | |pSB1C3<br> | ||
| + | |- | ||
| + | |'''Submitted by''' | ||
| + | |[http://2013.igem.org/Team:Freiburg Freiburg 2013] | ||
| + | |} | ||
| + | |||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered [https://parts.igem.org/Part:BBa_K1150000 dCas9] protein to the Bla locus target 4 . This RNaimer is bulid of the uniCAS [https://parts.igem.org/Part:BBa_K1150034 RNAimer] and contains the DNA sequence coding for a crRNA that binds complementary to Bla 4 target. | ||
| − | <!-- | + | |
| + | Freiburg 2013 used this plasmid to test the efficiency of target Bla4 by activating or repressing a SEAP reporter gene. | ||
| + | |||
| + | <!-- | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1150042 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1150042 SequenceAndFeatures</partinfo> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Revision as of 23:14, 4 October 2013
uniCAS RNaimer to Bla Target 4
| RNaimer (Bla target 4) | |
|---|---|
| Function | Targeting VEGF4 with Cas9 |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Usage and Biology
This device contains loci coding for small RNAs that are able to target the catalytically-inactive, engineered dCas9 protein to the Bla locus target 4 . This RNaimer is bulid of the uniCAS RNAimer and contains the DNA sequence coding for a crRNA that binds complementary to Bla 4 target.
Freiburg 2013 used this plasmid to test the efficiency of target Bla4 by activating or repressing a SEAP reporter gene.