Difference between revisions of "Part:BBa K1041004"

Latest revision as of 23:11, 4 October 2013

AntG Promoter + Gus gene

Team NRP-UEA_Norwich 2013 desgined this part using biobrick BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene would be excised from BBa_K1041001 and a gene encoding Gus ligated downstream of the promoter of BBa_K1041001 to create a new biobrick. The protein product of the GUS gene provides a reporter that can indicate expression of the gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 498
Illegal NgoMIV site found at 630
Illegal NgoMIV site found at 1227
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 896

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 __NOTOC__
 <partinfo>BBa_K1041004 short</partinfo>
-Team NRP-UEA_Norwich 2013 desgined this part using biobricks BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041002 and a gene encoding Gus ligated in front of the promoter of BBa_K1041001 to create a new biobrick.
+Team NRP-UEA_Norwich 2013 desgined this part using biobrick BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene would be excised from BBa_K1041001 and a gene encoding Gus ligated downstream of the promoter of BBa_K1041001 to create a new biobrick. The protein product of the GUS gene provides a reporter that can indicate expression of the gene.
 <!-- Add more about the biology of this part here