Difference between revisions of "Part:BBa K1122680"
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Part was cloned from ''B. subtilis'' gDNA. Reaction product was analysed on an agarose gel (Figure 1) | Part was cloned from ''B. subtilis'' gDNA. Reaction product was analysed on an agarose gel (Figure 1) | ||
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Figure 1. Agarose gel shows a PCR product of a desired size | Figure 1. Agarose gel shows a PCR product of a desired size | ||
Latest revision as of 22:45, 4 October 2013
Plac - LacZ - Fur
This part enables expression of ferric uptake repressor (Fur) using IPTG induction
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 769
Cloning
Part was cloned from B. subtilis gDNA. Reaction product was analysed on an agarose gel (Figure 1)
Figure 1. Agarose gel shows a PCR product of a desired size
Functional analysis
It was assumed that over-expression of Fur could increase bacterial tolerance to increased iron concentration due to enhanced perception of environmental [iron]. This was analysed experimentally be culturing cells induced and not induced to express Fur in LB nmedium supplemented with increasing concentrations of iron. Results obtained (figure 2)indicate that indeed the cells that contain more copies of Fur protein survive better in medium with increased iron levels
Figure 2. Survival of cells induce and noninduced to express Fur in varying iron concentrations.