Difference between revisions of "Part:BBa K1150035"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1150035 short</partinfo>
 
<partinfo>BBa_K1150035 short</partinfo>
  
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#FFBF00;"|RNAimer (EMX1 target)
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|-
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|'''Function'''
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|Targeting EMX1 with Cas9
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|-
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|'''Use in'''
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|Mammalians
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Backbone'''
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|pSB1C3<br>
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|-
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|'''Submitted by'''
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|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
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|}
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This device derived from [https://parts.igem.org/Part:BBa_K1150034 BBa_K1150034] can be used in combination with all devices with [https://parts.igem.org/Part:BBa_K1150000 dCas9]. It contains a DNA sequence coding for a crRNA that binds complementary to EMX1 target (GGAAGGGCCTGAGTCCGAGCAGAAGAAGAA).
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For more information about dCas9 devices [http://2013.igem.org/Team:Freiburg klick here].
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==Biology and Usage==
  
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Freiburg 2013 used this plasmid to test the efficiency of target EMX1 by activating or repressing a SEAP reporter gene.
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===Usage and Biology===
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Latest revision as of 22:39, 4 October 2013

uniCAS RNAimer to EMX1

RNAimer (EMX1 target)
Function Targeting EMX1 with Cas9
Use in Mammalians
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

This device derived from BBa_K1150034 can be used in combination with all devices with dCas9. It contains a DNA sequence coding for a crRNA that binds complementary to EMX1 target (GGAAGGGCCTGAGTCCGAGCAGAAGAAGAA).

For more information about dCas9 devices [http://2013.igem.org/Team:Freiburg klick here].

Biology and Usage

Freiburg 2013 used this plasmid to test the efficiency of target EMX1 by activating or repressing a SEAP reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216