Difference between revisions of "Part:BBa K1150039"
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| − | This device can be used in combination with dCas9. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF4 target (GTGAGTGTGTGCGTGTGGGGTTGAGGGCGT). | + | This device derived from [https://parts.igem.org/Part:BBa_K1150034 BBa_K1150034] can be used in combination with all devices with [https://parts.igem.org/Part:BBa_K1150000 dCas9]. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF4 target (GTGAGTGTGTGCGTGTGGGGTTGAGGGCGT). |
| − | + | For more information about dCas9 devices [http://2013.igem.org/Team:Freiburg klick here]. | |
| − | + | ==Biology and Usage== | |
| + | Freiburg 2013 used this plasmid to test the efficiency of target VEGF4 by activating or repressing a SEAP reporter gene. | ||
<span class='h3bb'> | <span class='h3bb'> | ||
==Sequence and Features== | ==Sequence and Features== | ||
Revision as of 22:29, 4 October 2013
uniCAS RNAimer to target VEGF4
| RNAimer (VEGF4 target) | |
|---|---|
| Function | Targeting VEGF4 with Cas9 |
| Use in | Mammalians |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
This device derived from BBa_K1150034 can be used in combination with all devices with dCas9. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF4 target (GTGAGTGTGTGCGTGTGGGGTTGAGGGCGT).
For more information about dCas9 devices [http://2013.igem.org/Team:Freiburg klick here].
Biology and Usage
Freiburg 2013 used this plasmid to test the efficiency of target VEGF4 by activating or repressing a SEAP reporter gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 224
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 216
Literature