Difference between revisions of "Part:BBa K1150010"

Latest revision as of 22:00, 4 October 2013

NLS

NLS
Function	nuclear localization signal
Use in	Mammalian cells
RFC standard	RFC 25
Backbone	pSB1C3
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

The nuclear localization signal or nuclear localization sequence (NLS) is a specific amino acid sequence in nuclear proteins.
The NLS is recognized by importins, which enable the nuclear proteins the access to the nucleus.
It consists mostly of positively charged amino acids like lysine and arginine.

In our constructs the NLS found place in many proteins such as dCas9.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
-! colspan="2" style="background:#FFBF00;"|dCas9
+! colspan="2" style="background:#FFBF00;"|NLS
 |-
 |'''Function'''
@@ Line 22: / Line 22: @@
 The nuclear localization signal or nuclear localization sequence (NLS) is a specific amino acid sequence in nuclear proteins. <br> The NLS is recognized by importins, which enable the nuclear proteins the access to the nucleus. <br> It consists mostly of positively charged amino acids like lysine and arginine. <br><br>
-In our constructs the NLS found place in many proteins such as [https://parts.igem.org/Part:BBa_K1150000 Cas9].
+In our constructs the NLS found place in many proteins such as [https://parts.igem.org/Part:BBa_K1150000 dCas9].
 <!-- Add more about the biology of this part here