Difference between revisions of "Part:BBa K1150010"
 
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! colspan="2" style="background:#FFBF00;"|dCas9
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! colspan="2" style="background:#FFBF00;"|NLS
 
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The nuclear localization signal or nuclear localization sequence (NLS) is a specific amino acid sequence in nuclear proteins. <br> The NLS is recognized by importines, which enable the nuclear proteins the access to the nucleus. <br> It consists mostly of positively charged amino acids like lysine and arginine. <br><br>
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The nuclear localization signal or nuclear localization sequence (NLS) is a specific amino acid sequence in nuclear proteins. <br> The NLS is recognized by importins, which enable the nuclear proteins the access to the nucleus. <br> It consists mostly of positively charged amino acids like lysine and arginine. <br><br>
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In our constructs the NLS found place in many proteins such as Cas9.  
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In our constructs the NLS found place in many proteins such as [https://parts.igem.org/Part:BBa_K1150000 dCas9].
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==Sequence and Features==</span>
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==Sequence and Features==
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<partinfo>BBa_K1150010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1150010 SequenceAndFeatures</partinfo>
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Latest revision as of 22:00, 4 October 2013

NLS

NLS
Function nuclear localization signal
Use in Mammalian cells
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

The nuclear localization signal or nuclear localization sequence (NLS) is a specific amino acid sequence in nuclear proteins.
The NLS is recognized by importins, which enable the nuclear proteins the access to the nucleus.
It consists mostly of positively charged amino acids like lysine and arginine.

In our constructs the NLS found place in many proteins such as dCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]