Difference between revisions of "Part:BBa K1055000:Experience"

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(→‎Applications of BBa_K1055000)
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''Characterization mKate''
 
''Characterization mKate''
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[[Image:MKate-LSSmOrange_FRET_Pair.png|400px|thumb|Figure 2. '''Overlay of excitation Spectrum (dashed line) and emission spectrum (solid line) of mKate and LSSmOrange ''']]
  
 
mKate2 is a red fluorescent protein that we want to use as a acceptor for FRET. According to Evrogen [2] mKate2 has an excitation maximum at 588 nm and an emission maximum at 633 nm. The emission maximum is big enough to get stimulated by [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1055001 LSSmOrange], the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement.  
 
mKate2 is a red fluorescent protein that we want to use as a acceptor for FRET. According to Evrogen [2] mKate2 has an excitation maximum at 588 nm and an emission maximum at 633 nm. The emission maximum is big enough to get stimulated by [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1055001 LSSmOrange], the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement.  
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[[Image:Spectra_mKate.png|400px|thumb|Figure 1. '''Excitation Spectrum (dashed line) and emission spectrum (solid line) of mKate with marked maximums''' ]]
 
[[Image:Spectra_mKate.png|400px|thumb|Figure 1. '''Excitation Spectrum (dashed line) and emission spectrum (solid line) of mKate with marked maximums''' ]]
  
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[[Image:MKate-LSSmOrange_FRET_Pair.png|400px|thumb|Figure 1. '''Overlay of excitation Spectrum (dashed line) and emission spectrum (solid line) of mKate and LSSmOrange ''']]
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[[Image:mKate_bac.png|400px|thumb|Figure 3. '''Bl21-DE3 cells with mKate ''']]
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[[Image:mKate_bac.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
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[[Image:Grow_log.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
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[[Image:Grow_log.png|400px|thumb|Figure 4. '''Semi-logarithmic growth curves of BL21DE3 WT, BL21DE3 mKate and BL21DE3 LSSmOrange. The ln (OD600 values) are plotted against the time [min].''']]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 21:59, 4 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1055000

Characterization mKate

Figure 2. Overlay of excitation Spectrum (dashed line) and emission spectrum (solid line) of mKate and LSSmOrange

mKate2 is a red fluorescent protein that we want to use as a acceptor for FRET. According to Evrogen [2] mKate2 has an excitation maximum at 588 nm and an emission maximum at 633 nm. The emission maximum is big enough to get stimulated by LSSmOrange, the other fluorescent protein. Of course, we checked also the emission and excitation of our BL21(DE3) cells (data not shown) and we can eliminate the theories that these cells disturb this florescence measurement.


Figure 1. Excitation Spectrum (dashed line) and emission spectrum (solid line) of mKate with marked maximums
Figure 3. Bl21-DE3 cells with mKate
Figure 4. Semi-logarithmic growth curves of BL21DE3 WT, BL21DE3 mKate and BL21DE3 LSSmOrange. The ln (OD600 values) are plotted against the time [min].

User Reviews

UNIQ80dba6040d9d67b8-partinfo-00000000-QINU UNIQ80dba6040d9d67b8-partinfo-00000001-QINU