Difference between revisions of "Help:Plasmid backbones"

m
m
Line 4: Line 4:
 
|valign='top' width=600px|
 
|valign='top' width=600px|
 
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmids] are circular, double-stranded units of DNA that replicate within a cell independent of the chromosomal DNA within the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell]. They are often acquired by cells and can bestow the benefit of antibiotic resistance or protein production. Plasmids are used to incorporate biobrick devices and systems within cells via transformation.  
 
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid Plasmids] are circular, double-stranded units of DNA that replicate within a cell independent of the chromosomal DNA within the [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell cell]. They are often acquired by cells and can bestow the benefit of antibiotic resistance or protein production. Plasmids are used to incorporate biobrick devices and systems within cells via transformation.  
−
 
−
*'''[[Help:Plasmids/Nomenclature | Plasmid Naming]]''' - Information about our naming conventions for BioBrick compatible vectors (like pSB1AC3).
 
  
 
*'''[[Help:Plasmids/Construction Plasmids| Types of Plasmids]]''' - What's a construction plasmid? Does "plasmid" mean vector backbone only?
 
*'''[[Help:Plasmids/Construction Plasmids| Types of Plasmids]]''' - What's a construction plasmid? Does "plasmid" mean vector backbone only?
Line 11: Line 9:
 
*'''[[Help:Plasmid features|Plasmid features]]''' - Features of plasmid parts & their  feature tables of plasmids mean
 
*'''[[Help:Plasmid features|Plasmid features]]''' - Features of plasmid parts & their  feature tables of plasmids mean
  
−
*Browse [https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid plasmid parts][[Image:Part icon plasmid.png]]
+
*'''[[Help:Plasmids/Nomenclature | Plasmid Naming]]''' - Information about our naming conventions for BioBrick compatible vectors (like pSB1AC3).
 +
 
 +
*[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=Plasmid '''Browse plasmid parts'''][[Image:Part icon plasmid.png]]
  
 
|-
 
|-

Revision as of 20:03, 4 August 2006

PlasmidDNA.png

Plasmids are circular, double-stranded units of DNA that replicate within a cell independent of the chromosomal DNA within the cell. They are often acquired by cells and can bestow the benefit of antibiotic resistance or protein production. Plasmids are used to incorporate biobrick devices and systems within cells via transformation.

  • Types of Plasmids - What's a construction plasmid? Does "plasmid" mean vector backbone only?
  • Plasmid features - Features of plasmid parts & their feature tables of plasmids mean
  • Plasmid Naming - Information about our naming conventions for BioBrick compatible vectors (like pSB1AC3).

Antibiotic Resistance

By incorporating an antibiotic-resistance gene on a plasmid, engineered-cells are able to be selected for using the appropriate antibiotic. The most common resistances are ampicillin ("Amp"), kanomycin ("Kan"), tetracycline ("Tet") and chloramphenicol ("Chlor"). For more information on which plasmids carry these resistances, click the nomenclature documentation section.

All of our plasmids are available through the use of BBa_P1010. (Information on how to use that part is available on its part page)