Difference between revisions of "Part:BBa K1150010"
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| − | The nuclear localization signal or nuclear localization sequence (NLS) is a specific amino acid sequence in nuclear proteins. <br> The NLS is recognized by | + | The nuclear localization signal or nuclear localization sequence (NLS) is a specific amino acid sequence in nuclear proteins. <br> The NLS is recognized by importins, which enable the nuclear proteins the access to the nucleus. <br> It consists mostly of positively charged amino acids like lysine and arginine. <br><br> |
| − | In our constructs the NLS found place in many proteins such as Cas9. | + | In our constructs the NLS found place in many proteins such as [https://parts.igem.org/Part:BBa_K1150000 Cas9]. |
| − | + | ||
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Revision as of 21:26, 4 October 2013
NLS
| dCas9 | |
|---|---|
| Function | nuclear localization signal |
| Use in | Mammalian cells |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
The nuclear localization signal or nuclear localization sequence (NLS) is a specific amino acid sequence in nuclear proteins.
The NLS is recognized by importins, which enable the nuclear proteins the access to the nucleus.
It consists mostly of positively charged amino acids like lysine and arginine.
In our constructs the NLS found place in many proteins such as Cas9.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]