Difference between revisions of "Part:BBa K1033121"
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Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". | Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum". | ||
Revision as of 19:56, 4 October 2013
B0034-ExpTag-Linker-Miraculin-Linker-6xHis
Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum".
This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
Applications
Miraculin can be used as a sweetener.
Characterization data from Uppsala iGEM 2013
We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]