Difference between revisions of "Part:BBa K1033121"
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__NOTOC__
 
__NOTOC__
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<partinfo>Miraculin</partinfo>
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<partinfo>BBa_K1033120 short</partinfo>
  
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Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum", this part is a synthetic construct.
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Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum".
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This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
  
 
'''Applications'''
 
'''Applications'''

Revision as of 19:34, 4 October 2013

B0034-ExpTag-Linker-Miraculin-Linker-6xHis

Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum".

This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Applications

Miraculin can be used as a sweetener.


Characterization data from Uppsala iGEM 2013

We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]