Difference between revisions of "Part:BBa K1033121"
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<partinfo>BBa_K1033121 short</partinfo>
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<partinfo>BBa_K1033120 short</partinfo>
  
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None, this construct contains no RBS or promoter
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Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum".
  
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== Results ==
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This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.
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We were successful in both subcloning the synthetic construct to a desired plasmid and isolating the Maraculin gene by itself. The sequencencing was done at GATC biotch and Uppsala Genome center. We were not successful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.  
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'''Applications'''
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Miraculin can be used as a sweetener.
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==Characterization data from Uppsala iGEM 2013==
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We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1033121 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1033120 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1033121 parameters</partinfo>
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<partinfo>BBa_K1033120 parameters</partinfo>
 
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Revision as of 19:16, 4 October 2013

B0034-ExpTag-Linker-Miraculin-Linker-6xHis

Construct needed to produce the protein miraculin, responsible for the taste-altering effects of the berries from the plant "Synsepalum Dulcificum".

This part is a synthetic construct, fitted with an export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes.

Applications

Miraculin can be used as a sweetener.


Characterization data from Uppsala iGEM 2013

We were not sucessful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]