Difference between revisions of "Part:BBa K1033121"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1033121 short</partinfo>
 
<partinfo>BBa_K1033121 short</partinfo>
  
 
None, this construct contains no RBS or promoter
 
None, this construct contains no RBS or promoter
 +
 +
 +
== Results ==
 +
We were successful in both subcloning the synthetic construct to a desired plasmid and isolating the Maraculin gene by itself. The sequencencing was done at GATC biotch and Uppsala Genome center. We were not successful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:59, 4 October 2013

Miraculin

None, this construct contains no RBS or promoter


Results

We were successful in both subcloning the synthetic construct to a desired plasmid and isolating the Maraculin gene by itself. The sequencencing was done at GATC biotch and Uppsala Genome center. We were not successful in either assembling or mutagenizing a promoter to the construct, and as such could not characterize it further.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]