Difference between revisions of "Part:BBa K1045010:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | For cloning of the inverse RBS [[Part:BBa_K1045010|BBa_K1045010]], hybridization oligos were used. Prefix and Suffix were switched to yield an inverted RBS. | + | For cloning of the inverse RBS [[Part:BBa_K1045010|BBa_K1045010]], hybridization oligos for the RBS [[Part:BBa_B0034|BBa_B0034]] were used. Prefix and Suffix were switched to yield an inverted RBS. |
===Source=== | ===Source=== | ||
Revision as of 18:49, 4 October 2013
RBS BBa_B0034 with inversed Pre- and Suffix
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For cloning of the inverse RBS BBa_K1045010, hybridization oligos for the RBS BBa_B0034 were used. Prefix and Suffix were switched to yield an inverted RBS.
Source
The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of BBa_B0034.