Difference between revisions of "Part:BBa K1123001"
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===Characterization===
 
===Characterization===
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This part is a composite part which is comprised of a number of other important parts namely [[Part: BBa_K1123000 | BBa_K1123000]] and [[Part: BBa_K1123013 | BBa_K1123013]]
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This part is a composite part which is comprised of a number of other important parts namely [[Part: BBa_K1123000 | BBa_K1123000]] and [[Part: BBa_K1123013 | BBa_K1123013]].
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This part has, in itself not been tested by us here at the TU-Eindhoven during the 2013 iGEM competition. However, as the other, important parts which make up this part have been tested, shown to work, and characterized we would like o refer you to these parts for a complete overview of the characterization of this part.
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We do not think there will be a change in the functionality of any of the separate parts when combining them. A similar composite part [[Part: BBa_K1123005 | BBa_K1123005]] was combined and tested and showed no change in functionality other than a decrease in expression.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 17:53, 4 October 2013

Protamine-1-Optimized Protein Production Construct

This composite part has been built up from a number of separate BioBricks, the most prominent of which the FNR promotor and the Protamine-1-Optimized Protein sequence. The FNR promotor sequence enables us to bring the Protamine-1-Optimized protein to expression anaerobically. Almost all E.coli strains posses a form of this promotor to induce metabolic processes once the bacteria enters anaerobic regions. The Protamine-1-Optimized protein is a human protein which we are expressing with the sole purpose of using it as a CEST contrast agent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 39
    Illegal XhoI site found at 359
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

This part is a composite part which is comprised of a number of other important parts namely BBa_K1123000 and BBa_K1123013.

This part has, in itself not been tested by us here at the TU-Eindhoven during the 2013 iGEM competition. However, as the other, important parts which make up this part have been tested, shown to work, and characterized we would like o refer you to these parts for a complete overview of the characterization of this part.

We do not think there will be a change in the functionality of any of the separate parts when combining them. A similar composite part BBa_K1123005 was combined and tested and showed no change in functionality other than a decrease in expression.