Difference between revisions of "Part:BBa K1093001"

Revision as of 16:58, 4 October 2013

sfYFP

Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm

Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908).

Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

-	Samples			Arithmetic mean	Standard deviation
sfGFP	100,60	100,37	99,02	100,00	0,85
sfCFP	17,95	18,38	17,36	17,90	0,51
sfBFP	40,65	41,52	42,25	41,47	0,80
sfYFP	218,87	216,51	217,87	217,75	1,19

Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

Also Team Warsaw 2013 measured SYFP2 (BBa_K864100) and compared it with sfYFP.

Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).

-	Samples			Arithmetic mean	Standard deviation
sfGFP	100,60	100,37	99,02	100,00	0,85
sfYFP	218,87	216,51	217,87	217,75	1,19
SYFP2	424,70	418,26	412,40	418,45	6,15

Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 13

@@ Line 5: / Line 5: @@
 Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908).
-https://static.igem.org/mediawiki/2013/7/70/Superfolderwykres.jpg
+[[File:Superfolderwykres.jpg|left|594px|border|caption]]
 ''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''
@@ Line 47: / Line 47: @@
 Also Team Warsaw 2013 measured SYFP2 (BBa_K864100) and compared it with sfYFP.
-https://static.igem.org/mediawiki/2013/3/31/Sfyfpsyfp2wykres.jpg
+[[File:Sfyfpsyfp2wykres.jpg|left|541px|border|caption]]
 ''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''