Difference between revisions of "Part:BBa K1093001"
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<partinfo>BBa_K1093001 short</partinfo>
 
<partinfo>BBa_K1093001 short</partinfo>
  
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Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence.
+
Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm
 +
 
 +
Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908).
 +
https://static.igem.org/mediawiki/2013/7/70/Superfolderwykres.jpg
 +
''Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''
 +
 
 +
{| class="wikitable"
 +
|-
 +
! scope="col"| -
 +
! colspan="3" | Samples
 +
! scope="col" | Arithmetic mean
 +
! scope="col" | Standard deviation
 +
|-
 +
|sfGFP
 +
|100,60
 +
|100,37
 +
|99,02
 +
|100,00
 +
|0,85
 +
|-
 +
|sfCFP
 +
|17,95
 +
|18,38
 +
|17,36
 +
|17,90
 +
|0,51
 +
|-
 +
|sfBFP
 +
|40,65
 +
|41,52
 +
|42,25
 +
|41,47
 +
|0,80
 +
|-
 +
|sfYFP
 +
|218,87
 +
|216,51
 +
|217,87
 +
|217,75
 +
|1,19
 +
|-
 +
|}
 +
''Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).''
 +
 
 +
Also Team Warsaw 2013 measured SYFP2 (BBa_K864100) and compared it with sfYFP.
 +
https://static.igem.org/mediawiki/2013/3/31/Sfyfpsyfp2wykres.jpg
 +
''Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''
 +
 
 +
{| class="wikitable"
 +
|-
 +
! scope="col"| -
 +
! colspan="3" | Samples
 +
! scope="col" | Arithmetic mean
 +
! scope="col" | Standard deviation
 +
|-
 +
|sfGFP
 +
|100,60
 +
|100,37
 +
|99,02
 +
|100,00
 +
|0,85
 +
|-
 +
|sfYFP
 +
|218,87
 +
|216,51
 +
|217,87
 +
|217,75
 +
|1,19
 +
|-
 +
|SYFP2
 +
|424,70
 +
|418,26
 +
|412,40
 +
|418,45
 +
|6,15
 +
|-
 +
|}
 +
''Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).''
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
 +
sfYFP can be used as reporter.
  
 
<!-- -->
 
<!-- -->

Revision as of 16:56, 4 October 2013

sfYFP

Superfolder Yellow Fluorescent Protein is a derivative of superfolder Green Fluorescent protein. Compared to eYFP it displays improved stability and strong yellow fluorescence. Excitation peak: 503 nm Emission peak: 540 nm

Team Warsaw 2013 measured sfYFP and other designed superfolder proteins and compared them to sfGFP (BBa_I746908). Superfolderwykres.jpg Fig 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

- Samples Arithmetic mean Standard deviation
sfGFP 100,60 100,37 99,02 100,00 0,85
sfCFP 17,95 18,38 17,36 17,90 0,51
sfBFP 40,65 41,52 42,25 41,47 0,80
sfYFP 218,87 216,51 217,87 217,75 1,19

Tab 1. – Fluorescence of sfCFP, sfYFP and sfBFP compared to sfGFP (BBa_I746908).

Also Team Warsaw 2013 measured SYFP2 (BBa_K864100) and compared it with sfYFP. Sfyfpsyfp2wykres.jpg Fig 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).

- Samples Arithmetic mean Standard deviation
sfGFP 100,60 100,37 99,02 100,00 0,85
sfYFP 218,87 216,51 217,87 217,75 1,19
SYFP2 424,70 418,26 412,40 418,45 6,15

Tab 2. – Fluorescence of sfYFP and SYFP2 compared to sfGFP (BBa_I746908).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13