Difference between revisions of "Part:BBa K1088027:Experience"

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<I>Patrick Rosendahl Andreassen</I>
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We investigated MG1655 strain carrying both BBa_K1088016 on pSB1K3 and BBa_K1088027 (CPS) by phase contrast microscopy and flow cytometry in order to evaluate whether there was a change in size or morphology compared to the wildtype (WT). As indicated by the forward side scatter measurements, there was a difference in size between CPS and MG1655 wildtype both before and after induction in late exponential phase (0.2% arabinose, 1 mM IPTG, 1 mM MgCl2) . Although both strains became small upon induction,the difference in size relation between the CPS and the wildtype remained. The difference in size was less clear during phase contract microscopy.
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Revision as of 16:01, 4 October 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1088027

User Reviews

UNIQ82d1e829d90a233e-partinfo-00000000-QINU UNIQ82d1e829d90a233e-partinfo-00000001-QINU

•••

Patrick Rosendahl Andreassen

We investigated MG1655 strain carrying both BBa_K1088016 on pSB1K3 and BBa_K1088027 (CPS) by phase contrast microscopy and flow cytometry in order to evaluate whether there was a change in size or morphology compared to the wildtype (WT). As indicated by the forward side scatter measurements, there was a difference in size between CPS and MG1655 wildtype both before and after induction in late exponential phase (0.2% arabinose, 1 mM IPTG, 1 mM MgCl2) . Although both strains became small upon induction,the difference in size relation between the CPS and the wildtype remained. The difference in size was less clear during phase contract microscopy.

SDU2013_Part_BBa_K1088016.png

•••

Patrick Rosendahl Andreassen

SDU2013_Characterization_Dxs_2.png

Growth curve of dxs devices. Cells were started at OD600=0.005 and incubated at 37oC and 180 rpm. 2 triplicates of MG1655 carrying no vector (WT), empty pSB1C3 vector (pSB1C3), pSB1C3-Plac-dxs(B.subtilis) (BBa_K1088011) (No LacI), pSB1C3-Pcon-lacI-Plac-dxs(B.subtilis) (BBa_K1088027) (LacI(N)), or pSB1C3-Pcon-lacI:LVA-Plac-dxs(B.subtilis) (BBa_K1088013) (LacI(LVA)). At time 2.5 hours one of each triplicate was induced with 1 mM IPTG. No growth change was observed.