Difference between revisions of "Part:BBa K1088016:Experience"
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| + | <partinfo>BBa_K1088016 AddReview 3</partinfo> | ||
| + | <I>Patrick Rosendahl Andreassen</I> | ||
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| + | We investigated MG1655 strain carrying both BBa_K1088016 on pSB1K3 and BBa_K1088027 (CPS) by phase contrast microscopy and flow cytometry in order to evaluate whether there was a change in size or morphology compared to the wildtype (WT). As indicated by the forward side scatter measurements, there was a difference in size between CPS and MG1655 wildtype both before and after induction in late exponential phase (0.2% arabinose, 1 mM IPTG, 1 mM MgCl2) . Although both strains became small upon induction,the difference in size relation between the CPS and the wildtype remained. The difference in size was less clear during phase contract microscopy. | ||
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| + | https://static.igem.org/mediawiki/2013/d/d0/SDU2013_Part_BBa_K1088016.png | ||
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Latest revision as of 16:00, 4 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1088016
User Reviews
UNIQ265ef58639436dd7-partinfo-00000000-QINU UNIQ265ef58639436dd7-partinfo-00000001-QINU
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Patrick Rosendahl Andreassen |
We investigated MG1655 strain carrying both BBa_K1088016 on pSB1K3 and BBa_K1088027 (CPS) by phase contrast microscopy and flow cytometry in order to evaluate whether there was a change in size or morphology compared to the wildtype (WT). As indicated by the forward side scatter measurements, there was a difference in size between CPS and MG1655 wildtype both before and after induction in late exponential phase (0.2% arabinose, 1 mM IPTG, 1 mM MgCl2) . Although both strains became small upon induction,the difference in size relation between the CPS and the wildtype remained. The difference in size was less clear during phase contract microscopy.
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