Difference between revisions of "Part:BBa K1088027"

 
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<partinfo>BBa_K1088027 short</partinfo>
 
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The purpose of the brick is to increase the flow through the MEP pathway upon addition of IPTG.  
 
The purpose of the brick is to increase the flow through the MEP pathway upon addition of IPTG.  
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A similar part (BBa_K1088026) with a GFP fused to Dxs, was used to prove that the part is IPTG inducible. See reporter fusion for details.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 15:11, 4 October 2013

Dxs from B. subtilis with lac promoter and lacI (IPTG inducible)

The part consist of the dxs gene derived from B. subtilis under the control of the lac promoter and has a strong RBS.

To repress expression from the lac promoter, the lacI gene under a constitutive promoter, with a strong RBS and a efficient terminator is placed counter-clockwise to the reporter fusion. The repression can then be relieved with addition of IPTG, which binds and inhibits the function of LacI.

The purpose of the brick is to increase the flow through the MEP pathway upon addition of IPTG.

A similar part (BBa_K1088026) with a GFP fused to Dxs, was used to prove that the part is IPTG inducible. See reporter fusion for details.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2460
    Illegal EcoRI site found at 3117
    Illegal PstI site found at 2518
    Illegal PstI site found at 2964
    Illegal NgoMIV site found at 2417
    Illegal AgeI site found at 2310
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2259
    Illegal SapI.rc site found at 2958