Difference between revisions of "Part:BBa K1111003:Design"

 
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===Design Notes===
 
===Design Notes===
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I took 500bp upstream from the beginning of the target gene
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The Promoter was isolated directly from the Genimic DNA by PCR. In order to have the entire sequence, we took the sequence 500bp upstream until the start codon.
−
 
+
−
 
+
  
 
===Source===
 
===Source===
 +
Escherichia Coli, K-12, Substrain MG1655
  
−
E.coli, Substrain MG1655
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===References===
 +
Journal of Bacteriology<BR>
 +
Nucleotode Sequence of the Escherichiea coli cad Operon: a system for neutralization of Low Extracellular pH <BR>
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Shi-Yuan meng and George N. Bennett<BR>
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J. Bacteriol 1992<BR>
  
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===References===
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Journal of Bacteriology <BR>
 +
Regulation of the Escherichia coli cad Operon: Location of a Site Required for Acid Induction <BR>
 +
Shi-Yuan meng and George N. Bennett <BR>
 +
J. Bacteriol 1992 <BR>

Latest revision as of 13:50, 4 October 2013


Cad Promoter induced by low pH and high lysine conentrations


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 474


Design Notes

The Promoter was isolated directly from the Genimic DNA by PCR. In order to have the entire sequence, we took the sequence 500bp upstream until the start codon.

Source

Escherichia Coli, K-12, Substrain MG1655

References

Journal of Bacteriology
Nucleotode Sequence of the Escherichiea coli cad Operon: a system for neutralization of Low Extracellular pH
Shi-Yuan meng and George N. Bennett
J. Bacteriol 1992

Journal of Bacteriology
Regulation of the Escherichia coli cad Operon: Location of a Site Required for Acid Induction
Shi-Yuan meng and George N. Bennett
J. Bacteriol 1992