Difference between revisions of "PBAD Promoter Family"
(→Overview) |
|||
| (5 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
| − | == | + | __NOTOC__ |
| + | <div class="noprint" style="padding: 10px; color: #ffffff; background-color: #C0C0C0; width: 680px; align: center"> | ||
| + | <center> | ||
| + | [[pBAD Promoter Family |Family:]] | ||
| + | [[Part:BBa_K206000 |pBAD strong]] | ||
| + | [[Part:BBa_I13453 |pBAD wild type]] | ||
| + | [[Part:BBa_K206001 |pBAD weak]] | ||
| + | </center> | ||
| + | </div> | ||
| + | =Overview= | ||
[http://2009.igem.org/Team:British_Columbia We] have designed and characterized a family of arabinose-inducible pBAD promoters, consisting of the following members: | [http://2009.igem.org/Team:British_Columbia We] have designed and characterized a family of arabinose-inducible pBAD promoters, consisting of the following members: | ||
| + | |||
| + | Note that a new set of pBAD promoters have been characterized; the [https://parts.igem.org/PBAD_SPL pBAD SPL] is a set of on non-leaky pBAD promoters with varying strength. | ||
{| | {| | ||
|<div style="text-align: center; valign: center; font-family: Arial; font-size: 12pt"> | |<div style="text-align: center; valign: center; font-family: Arial; font-size: 12pt"> | ||
| Line 10: | Line 21: | ||
<partinfo>K206001</partinfo>: pBAD weak | <partinfo>K206001</partinfo>: pBAD weak | ||
| + | [[Image:PBAD family - Sequences.png|center|frame|Alignment of the mutated bases in pBAD weak and pBAD strong compared to I13453.]] | ||
==Characterization== | ==Characterization== | ||
| − | [[Image:PBAD family - | + | See the individual parts pages for details on characterization. |
| + | ===Transfer Function=== | ||
| + | [[Image:PBAD family - Transfer Function.png|center|frame|Transfer functions relating input concentration to output for each promoter in the pBAD family.]] | ||
| + | ===Expression Rate=== | ||
| + | [[Image:PBAD family - Timecourse.png|center|frame|Rate of RFP expression for each promoter in the pBAD family.]] | ||
| + | ===Specificity=== | ||
| + | [[Image:PBAD family - Specificity.png|center|frame|All of the promoters in the pBAD family respond specifically to L-arabinose.]] | ||
Latest revision as of 13:38, 4 October 2013
Overview
[http://2009.igem.org/Team:British_Columbia We] have designed and characterized a family of arabinose-inducible pBAD promoters, consisting of the following members:
Note that a new set of pBAD promoters have been characterized; the pBAD SPL is a set of on non-leaky pBAD promoters with varying strength.
