Difference between revisions of "Part:BBa K1104100"
 
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<partinfo>BBa_K1104100 short</partinfo>
 
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==Introduction==
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mngB, a passage of gene in ''Escherichia coli'' K-12 substrain MG1655, can produce alpha-mannosidase, which is involved in the cleavage of the alpha form of mannose. The alpha-mannosidase reaction is to turn 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate.
  
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mngB, a passage of gene in ''Escherichia coli'' K-12 substr MG1655, can produce alpha-mannosidase, which is involved in the cleavage of the alpha form of mannose. The alpha-mannosidase reaction is to turn 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate.
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==Usage and Biology==
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The mannose degradation pathway is often seen in many organisms, such as the reverse reaction of the post-translation (glycosylation) on polar filaments of Fungi. The reason why we choose mannosidase gene from MG1655 is that this species of ''E-coli'' are able to live in bees' midgut. That way, this enzyme can well perform in our ''Bee. coli''.  We tend to overexpress this gene in order to inhibit the production of the polar protein in the sprouting of ''Nosema. ceranae'', the target fungi that we are eager to eliminate.
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===Usage and Biology===
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The mannose degradation pathway is often seen in many organisms, and the reason why we choose from MG1655 is that this species of ''E-coli'' are able to live in bees' midgut. Besides MG1655 is also the bacteria that we are going to transform our whole circuit into.  This decision make our experiment more easier and more certain that this enzyme can well perform in our ''Bee. coli''.  We tent to overexpress this gene in order to inhibit the production of the PTP1 in the polar filament of ''N. ceranae'', the chosen target fungi that we are eager to eliminate.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 13:27, 4 October 2013

mngB - alpha-mannosidase

Introduction

mngB, a passage of gene in Escherichia coli K-12 substrain MG1655, can produce alpha-mannosidase, which is involved in the cleavage of the alpha form of mannose. The alpha-mannosidase reaction is to turn 2-O-(6-phospho-α-mannosyl)-D-glycerate into mannose-6-phosphate and glycerate.

Usage and Biology

The mannose degradation pathway is often seen in many organisms, such as the reverse reaction of the post-translation (glycosylation) on polar filaments of Fungi. The reason why we choose mannosidase gene from MG1655 is that this species of E-coli are able to live in bees' midgut. That way, this enzyme can well perform in our Bee. coli. We tend to overexpress this gene in order to inhibit the production of the polar protein in the sprouting of Nosema. ceranae, the target fungi that we are eager to eliminate. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 716
    Illegal BglII site found at 992
    Illegal BglII site found at 2139
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 235
    Illegal AgeI site found at 2242
  • 1000
    COMPATIBLE WITH RFC[1000]