Difference between revisions of "Part:BBa K1028001"

Revision as of 12:27, 4 October 2013

INP.Si4 Silica Binding Complex
http://i276.photobucket.com/albums/kk29/JosephBeetBeet/ff81cf4b-7969-4340-9a16-24e76dc3e5e8.jpg
This biobrick was designed utilise Ice Nucleation Protein to express the Si4 Binding Peptide on the extracellular surface of the plasma membrane of E.coli cells.

Usage and Experimental Data

Fig.1: Monitoring the growth rate of cells containing Si4 Binding Peptide within the PSB1C3 standard iGEM submission plasmid. Although the graph shows a log and a lag phase for the cells containing the insert plasmid, the absorbance reached in log phase is very low compared to the control cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Unknown
21
INCOMPATIBLE WITH RFC[21]
Unknown
23
INCOMPATIBLE WITH RFC[23]
Unknown
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 409
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 http://i276.photobucket.com/albums/kk29/JosephBeetBeet/ff81cf4b-7969-4340-9a16-24e76dc3e5e8.jpg
 <br>
-Fusion of the silica binding peptide Si4, part BBa_K1028000, to Ice Nucleation Protein (INP) to achieve presentation and expression of the binding domain on the outer membrane.
+This biobrick was designed utilise Ice Nucleation Protein to express the Si4 Binding Peptide on the extracellular surface of the plasma membrane of E.coli cells.
+=== '''Usage and Experimental Data''' ===
+<html>
+<img src="https://static.igem.org/mediawiki/2013/c/cc/Leeds_BS2Growthcurve.png" style="width:500px;margin-right:160px"  ></a>
+<p style="text-align:center"><b>Fig.1:</b> Monitoring the growth rate of cells containing Si4 Binding Peptide within the PSB1C3 standard iGEM submission plasmid. Although the graph shows a log and a lag phase for the cells containing the insert plasmid, the absorbance reached in log phase is very low compared to the control cells.
+</p></html>
 <!-- Add more about the biology of this part here