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==Literature==
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==References==
 
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[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332
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Revision as of 12:13, 4 October 2013

uniCAS UV Light Switch Part II - Repressor

uniCAS UV Light Switch Part II - Repressor
Function Repression domain of UVB light

induced gene expression control

Use in Mammalians
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

The fusion protein COP1-KRAB is an interaction partner of dCas9-UVR8. When crRNA and tracrRNA bind to Cas9, the protein is able to bind complementary DNA. If the system is exposed to UVB light (311 nm), the UVR8 receptor changes its configuration and recruits this COP1-KRAB protein. This represses gene expression induced by UVB light.


Biology and Usage

E3-ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC 1 (COP1) is the interaction partner of ultraviolet B (UVB) light receptor UVR8 [1]. When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Then it recruits COP1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1373
    Illegal BsaI.rc site found at 1450
    Illegal BsaI.rc site found at 1876
    Illegal SapI.rc site found at 962
    Illegal SapI.rc site found at 1840

References

[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332