Difference between revisions of "Part:BBa K1150030"
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| − | The fusion protein COP1-VP16 is an interaction partner of [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8]. It's a part of the UVB light controlled mechanism to activate or repress gene expression. First the dCas9-UVR8 fusion protein binds specifically to a DNA target sequence. If the system is exposed to UVB light (311 nm), the UVR8 receptor of UV changes its configuration and recruits this COP1-VP16 protein. This activates gene expression induced by UVB light. | + | The fusion protein COP1-VP16 is an interaction partner of [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8]. It's a part of the UVB light controlled mechanism to activate or repress gene expression. First the dCas9-UVR8 fusion protein binds specifically to a DNA target sequence. If the system is exposed to UVB light (311 nm), the UVR8 receptor of UV changes its configuration and recruits this COP1-VP16 protein. This activates gene expression induced by UVB light. Have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light project page]. |
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E3-ubiquitin ligase Constitutively Photomorphogenic 1 (COP1) is the interaction partner of ultraviolet B (UVB) light receptor [https://parts.igem.org/Part:BBa_K1150006 UVR8] [1]. When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Not till then it recruits COP1. | E3-ubiquitin ligase Constitutively Photomorphogenic 1 (COP1) is the interaction partner of ultraviolet B (UVB) light receptor [https://parts.igem.org/Part:BBa_K1150006 UVR8] [1]. When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Not till then it recruits COP1. | ||
| + | For this UVB light controled gene activation system you need a combination of these BioBricks: [https://parts.igem.org/Part:BBa_K1150029 Cas9-UVR8], [https://parts.igem.org/Part:BBa_K1150030 COP1-VP16] and [https://parts.igem.org/Part:BBa_K1150034 RNaimer]. | ||
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Revision as of 12:06, 4 October 2013
uniCAS UV Light Switch Part II - Activator
| CMV:COP1-VP16:BGH | |
|---|---|
| Function | Activation domain of UVB light
induced gene expression control |
| Use in | Mammalians |
| RFC standard | RFC 25 |
| Backbone | pSB1C3 |
| Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
The fusion protein COP1-VP16 is an interaction partner of dCas9-UVR8. It's a part of the UVB light controlled mechanism to activate or repress gene expression. First the dCas9-UVR8 fusion protein binds specifically to a DNA target sequence. If the system is exposed to UVB light (311 nm), the UVR8 receptor of UV changes its configuration and recruits this COP1-VP16 protein. This activates gene expression induced by UVB light. Have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light project page].
Biology and Usage
E3-ubiquitin ligase Constitutively Photomorphogenic 1 (COP1) is the interaction partner of ultraviolet B (UVB) light receptor UVR8 [1]. When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Not till then it recruits COP1. For this UVB light controled gene activation system you need a combination of these BioBricks: Cas9-UVR8, COP1-VP16 and RNaimer.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 576
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1373
Illegal BsaI.rc site found at 1450
Illegal SapI.rc site found at 962
Literature