Difference between revisions of "Part:BBa K1028000"

Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1028000 short</partinfo>
 
<partinfo>BBa_K1028000 short</partinfo>
This is a short peptide of 15 amino acids designed to bind with high affinity to silica and silica-like particles, designed as a tag to be attached to the C-terminus of polypeptides. This part was originally designed to be conjugated to the C-terminal of Ice Nucleation Protein allowing extracellular expression of the binding region
+
<br>
 +
This is a short peptide of 15 amino acids designed to act as a tag binding with high affinity to silica and silica-like particles. As a tag it can easily be added to the C-terminus of proteins using molecular biology techniques allowing the peptide to assist with purification or to serve other experimental processes related to silica binding.
 
<br>
 
<br>
 
http://i276.photobucket.com/albums/kk29/JosephBeetBeet/d9a54a14-fa9a-4957-b11b-95d7323de4bf.jpg
 
http://i276.photobucket.com/albums/kk29/JosephBeetBeet/d9a54a14-fa9a-4957-b11b-95d7323de4bf.jpg
 +
<br>
 +
This part was originally designed by the Leeds 2013 iGEM team to bind with silica microspheres, acting as pathogen analogues, when presented on the exterior of the plasma membrane via fusion with Ice Nucleation Protein.
 +
  
  

Revision as of 11:41, 4 October 2013

Silica binding peptide
This is a short peptide of 15 amino acids designed to act as a tag binding with high affinity to silica and silica-like particles. As a tag it can easily be added to the C-terminus of proteins using molecular biology techniques allowing the peptide to assist with purification or to serve other experimental processes related to silica binding.
http://i276.photobucket.com/albums/kk29/JosephBeetBeet/d9a54a14-fa9a-4957-b11b-95d7323de4bf.jpg
This part was originally designed by the Leeds 2013 iGEM team to bind with silica microspheres, acting as pathogen analogues, when presented on the exterior of the plasma membrane via fusion with Ice Nucleation Protein.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]